Incubation:Article Title: Synovial Stem Cells and Their Responses to the Porosity of Microfibrous Scaffold
Article Snippet: Immunostaining and dye staining For immunostaining, cells were fixed with 4% paraformaldehyde, permeabilized with 0.5% Triton X-100 (Sigma-Aldrich, Inc.), and blocked with 1% bovine serum albumin (BSA) (Sigma-Aldrich, Inc.). .. Samples were incubated with specific primary antibodies against Sox10 (R&D systems), Sox17 (R&D systems), Snail (Santa Cruz Biotechnology, Inc.), Pax-3/7 (Santa Cruz Biotechnology, Inc.), Slug (Santa Cruz Biotechnology, Inc.), vimentin (DAKO), NG2 (Millipore), S100 calcium binding protein B positive (S100β) (Sigma-Aldrich), neural filament-medium polypeptide (NFM) (Sigma-Aldrich, Inc.), glial fibrillary acidic protein (GFAP) (Millipore), neuronal class III β-Tubulin (TUJ1) (Chemicon), smooth muscle α-actin (SMA) (Epitomics, Inc), smooth muscle myosin heavy chain (SM-MHC) (Santa Cruz Biotechnology, Inc.), calponin-1 (CNN-1) (Epitomic, Inc.) and Collagen II (Chemicon) for 2 hours at room temperature, washed with PBS for 3 times, and incubated with appropriate Alexa 488- and/or Alexa 546-labeled secondary antibodies (Molecular Probes, Inc.). .. Nuclei were stained with DAPI (Invitrogen Corp.).
Article Title: Engineering Micro- and Nanostructured Biomaterials for Cell and Tissue Engineering
Article Snippet: For immunostaining, cells were fixed with 4% paraformaldehyde (PFA), permeabilized with 0.5% Triton X-100 (Sigma–Aldrich, Inc.), and blocked with 1% bovine serum albumin (BSA) (Sigma–Aldrich, Inc.). .. Samples were incubated with specific primary antibodies against Sox10 (R&D systems), Sox17 (R&D systems), Snail (Santa Cruz Biotechnology, Inc.), Pax-3/7 (Santa Cruz Biotechnology, Inc.), Slug (Santa Cruz Biotechnology, Inc.), vimentin (DAKO), NG2 (Millipore), S100 calcium binding protein B positive (S100β) (Sigma–Aldrich, Inc.), neural filament-medium polypeptide (NFM) (Sigma–Aldrich, Inc.), glial fibrillary acidic protein (GFAP) (Millipore), neuronal class III β-Tubulin (TUJ1) (Chemicon), smooth muscle α-actin (SMA) (Epitomics, Inc), smooth muscle myosin heavy chain (SM-MHC) (Santa Cruz Biotechnology, Inc.), calponin-1 (CNN-1) (Epitomic, Inc.) and Collagen II (Chemicon) for 2 h at room temperature, washed three times with PBS, and incubated with appropriate Alexa 488- and/or Alexa 546-labeled secondary antibodies (Molecular Probes, Inc.). .. Nuclei were stained with DAPI (Invitrogen Corp.).
Article Title: Synovial stem cells and their responses to the porosity of microfibrous scaffold.
Article Snippet: For immunostaining, cells were fixed with 4% paraformaldehyde (PFA), permeabilized with 0.5% Triton X-100 (Sigma–Aldrich, Inc.), and blocked with 1% bovine serum albumin (BSA) (Sigma–Aldrich, Inc.). .. Samples were incubated with specific primary antibodies against Sox10 (R&D systems), Sox17 (R&D systems), Snail (Santa Cruz Biotechnology, Inc.), Pax-3/7 (Santa Cruz Biotechnology, Inc.), Slug (Santa Cruz Biotechnology, Inc.), vimentin (DAKO), NG2 (Millipore), S100 calcium binding protein B positive (S100b) (Sigma–Aldrich, Inc.), neural filament-medium polypeptide (NFM) (Sigma–Aldrich, Inc.), glial fibrillary acidic protein (GFAP) (Millipore), neuronal class III b-Tubulin (TUJ1) (Chemicon), smooth muscle a-actin (SMA) (Epitomics, Inc), smooth muscle myosin heavy chain (SM-MHC) (Santa Cruz Biotechnology, Inc.), calponin-1 (CNN-1) (Epitomic, Inc.) and Collagen II (Chemicon) for 2 h at room temperature, washed three times with PBS, and incubated with appropriate Alexa 488- and/or Alexa 546-labeled secondary antibodies (Molecular Probes, Inc.). .. Nuclei were stained with DAPI (Invitrogen Corp.).
Binding Assay:Article Title: Synovial Stem Cells and Their Responses to the Porosity of Microfibrous Scaffold
Article Snippet: Immunostaining and dye staining For immunostaining, cells were fixed with 4% paraformaldehyde, permeabilized with 0.5% Triton X-100 (Sigma-Aldrich, Inc.), and blocked with 1% bovine serum albumin (BSA) (Sigma-Aldrich, Inc.). .. Samples were incubated with specific primary antibodies against Sox10 (R&D systems), Sox17 (R&D systems), Snail (Santa Cruz Biotechnology, Inc.), Pax-3/7 (Santa Cruz Biotechnology, Inc.), Slug (Santa Cruz Biotechnology, Inc.), vimentin (DAKO), NG2 (Millipore), S100 calcium binding protein B positive (S100β) (Sigma-Aldrich), neural filament-medium polypeptide (NFM) (Sigma-Aldrich, Inc.), glial fibrillary acidic protein (GFAP) (Millipore), neuronal class III β-Tubulin (TUJ1) (Chemicon), smooth muscle α-actin (SMA) (Epitomics, Inc), smooth muscle myosin heavy chain (SM-MHC) (Santa Cruz Biotechnology, Inc.), calponin-1 (CNN-1) (Epitomic, Inc.) and Collagen II (Chemicon) for 2 hours at room temperature, washed with PBS for 3 times, and incubated with appropriate Alexa 488- and/or Alexa 546-labeled secondary antibodies (Molecular Probes, Inc.). .. Nuclei were stained with DAPI (Invitrogen Corp.).
Article Title: Engineering Micro- and Nanostructured Biomaterials for Cell and Tissue Engineering
Article Snippet: For immunostaining, cells were fixed with 4% paraformaldehyde (PFA), permeabilized with 0.5% Triton X-100 (Sigma–Aldrich, Inc.), and blocked with 1% bovine serum albumin (BSA) (Sigma–Aldrich, Inc.). .. Samples were incubated with specific primary antibodies against Sox10 (R&D systems), Sox17 (R&D systems), Snail (Santa Cruz Biotechnology, Inc.), Pax-3/7 (Santa Cruz Biotechnology, Inc.), Slug (Santa Cruz Biotechnology, Inc.), vimentin (DAKO), NG2 (Millipore), S100 calcium binding protein B positive (S100β) (Sigma–Aldrich, Inc.), neural filament-medium polypeptide (NFM) (Sigma–Aldrich, Inc.), glial fibrillary acidic protein (GFAP) (Millipore), neuronal class III β-Tubulin (TUJ1) (Chemicon), smooth muscle α-actin (SMA) (Epitomics, Inc), smooth muscle myosin heavy chain (SM-MHC) (Santa Cruz Biotechnology, Inc.), calponin-1 (CNN-1) (Epitomic, Inc.) and Collagen II (Chemicon) for 2 h at room temperature, washed three times with PBS, and incubated with appropriate Alexa 488- and/or Alexa 546-labeled secondary antibodies (Molecular Probes, Inc.). .. Nuclei were stained with DAPI (Invitrogen Corp.).
Article Title: Synovial stem cells and their responses to the porosity of microfibrous scaffold.
Article Snippet: For immunostaining, cells were fixed with 4% paraformaldehyde (PFA), permeabilized with 0.5% Triton X-100 (Sigma–Aldrich, Inc.), and blocked with 1% bovine serum albumin (BSA) (Sigma–Aldrich, Inc.). .. Samples were incubated with specific primary antibodies against Sox10 (R&D systems), Sox17 (R&D systems), Snail (Santa Cruz Biotechnology, Inc.), Pax-3/7 (Santa Cruz Biotechnology, Inc.), Slug (Santa Cruz Biotechnology, Inc.), vimentin (DAKO), NG2 (Millipore), S100 calcium binding protein B positive (S100b) (Sigma–Aldrich, Inc.), neural filament-medium polypeptide (NFM) (Sigma–Aldrich, Inc.), glial fibrillary acidic protein (GFAP) (Millipore), neuronal class III b-Tubulin (TUJ1) (Chemicon), smooth muscle a-actin (SMA) (Epitomics, Inc), smooth muscle myosin heavy chain (SM-MHC) (Santa Cruz Biotechnology, Inc.), calponin-1 (CNN-1) (Epitomic, Inc.) and Collagen II (Chemicon) for 2 h at room temperature, washed three times with PBS, and incubated with appropriate Alexa 488- and/or Alexa 546-labeled secondary antibodies (Molecular Probes, Inc.). .. Nuclei were stained with DAPI (Invitrogen Corp.).
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